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Titlebook: DNA Vaccines; Methods and Protocol Monica Rinaldi,Daniela Fioretti,Sandra Iurescia Book 2014Latest edition Springer Science+Business Media

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發(fā)表于 2025-3-21 20:08:05 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱DNA Vaccines
副標題Methods and Protocol
編輯Monica Rinaldi,Daniela Fioretti,Sandra Iurescia
視頻videohttp://file.papertrans.cn/261/260210/260210.mp4
概述Includes cutting-edge methods and protocols involving DNA vaccine applications.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the exp
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: DNA Vaccines; Methods and Protocol Monica Rinaldi,Daniela Fioretti,Sandra Iurescia Book 2014Latest edition Springer Science+Business Media
描述.DNA Vaccines: Methods and Protocols, Third Edition .explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of DNA vaccines.? Featuring applications which should be of great value in moving vaccines from research to clinic, this detailed volume includes sections on DNA vaccine design and enhancement, delivery systems, production, purification, and quality, as well as chapters on new vaccine applications.? Written in the highly successful .Methods in Molecular Biology. series format, chapters contain introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .DNA Vaccines: Methods and Protocols, Third Edition. serves the important role of further documenting the potential of the DNA vaccination as a platform technology for treatment and prevention of human disease..
出版日期Book 2014Latest edition
關鍵詞DNA vaccination; Gene vaccines; Non-viral gene therapy; Production and purification; Vaccine design and
版次3
doihttps://doi.org/10.1007/978-1-4939-0410-5
isbn_softcover978-1-4939-4653-2
isbn_ebook978-1-4939-0410-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2014
The information of publication is updating

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沙發(fā)
發(fā)表于 2025-3-21 23:12:56 | 只看該作者
Strategies for Improving DNA Vaccine Performancestructure and easy manipulation, a wide range of strategies can be applied to improve DNA vaccine performance. This chapter discusses this topic in detail taking into account antigen/epitope selection and optimization, inclusion of intracellular targeting sequences and genetic adjuvants, and provision of T cell help.
板凳
發(fā)表于 2025-3-22 01:11:25 | 只看該作者
Ali Movaghar,Mansour Jamzad,Hossein Asadiep-by-step strategy for the construction of a plasmid vector with enhanced efficiency of nuclear plasmid uptake and the methodic for the in vivo efficiency evaluation of the obtained expression vector.
地板
發(fā)表于 2025-3-22 04:55:54 | 只看該作者
Rafa? Grycuk,Kelton Costa,Rafa? Scherer we describe fabrication methods of microneedles and DNA vaccine loading methods on the microneedle surface. Furthermore, in the latter part of this chapter, in vivo test protocols for assessing the efficacy of gene delivery using microneedles are described.
5#
發(fā)表于 2025-3-22 11:05:10 | 只看該作者
Enhancement of Plasmid-Mediated Transgene Expressionep-by-step strategy for the construction of a plasmid vector with enhanced efficiency of nuclear plasmid uptake and the methodic for the in vivo efficiency evaluation of the obtained expression vector.
6#
發(fā)表于 2025-3-22 16:22:08 | 只看該作者
7#
發(fā)表于 2025-3-22 20:00:49 | 只看該作者
1064-3745 ts.Contains key notes and implementation advice from the exp.DNA Vaccines: Methods and Protocols, Third Edition .explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of DNA vaccines.? Featuring applications which should be of great value in moving vaccine
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發(fā)表于 2025-3-23 00:03:15 | 只看該作者
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發(fā)表于 2025-3-23 02:08:29 | 只看該作者
Mostafa Borhani,Hassan Ghassemianaccines was tested firstly in vitro, in Chinese hamster ovary cells, through flow cytometry and real-time polymerase chain reaction analysis. Then, an essential in vivo study was performed in chickens, in order to evaluate the efficacy of DNA prototype vaccines, by measuring the antibody production by enzyme-linked immunosorbent assay.
10#
發(fā)表于 2025-3-23 06:27:43 | 只看該作者
https://doi.org/10.1007/978-981-97-0341-8how systemic and mucosal samples are prepared for analysis, followed by (4) two immunological assays: multicolor intracellular cytokine staining and tetramer/homing maker analysis that are used to evaluate effective systemic and mucosal T cell immunity.
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