Titlebook: DNA Topoisomerase Protocols; Volume I: DNA Topolo Mary-Ann Bjornsti,Neil Osheroff Book 1999 Humana Press 1999

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Two-Dimensional Agarose-Gel Electrophoresis of DNA Topoisomers,DNA molecules of totally different structures may have the same electrophoretic mobility. These DNA species can be resolved by two-dimensional (2-D) gel electrophoresis, which involves two successive operations carried out with one gel slab under different conditions and in orthogonal directions.

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Purification of Supercoiled Plasmid DNA,he capability of generating both positive and negative DNA supercoils during the process of transcription and replication, respectively. These supercoils must be relaxed in order for transcription and replication to continue unaffected, as outlined in preceding chapters. The DNA topoisomerases play

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Purification and Use of DNA Minicircles with Different Linking Numbers,information in topologically independent domains consisting of one or more genes and of all the elements required in cis for their functioning. The elements that belong to the same functional unit are topologically linked: they depend on each other and cooperate.

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Isolation of Knotted DNA from Coliphage P4,ssay for type II topoisomerase activity, even in crude extracts. Such assays have been useful in screening drugs that are suspected to inhibit type II topoisomerases. This chapter describes the isolation of knotted DNA from P4 phage. In the case of wild-type P4, such DNA molecules are double-strande

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Sedimentation Analysis of Bacterial Nucleoid Structure,f extracted nucleoids. Since the few DNA nicks that occur when nucleoids are isolated (.,.) are localized by the presence of 50–100 barriers to strand rotation (.,.), it is possible to recover chromosomal DNA in which most of each molecule is topologically constrained (.,.). Consequently, intracellu