Titlebook: DNA Topoisomerase Protocols; Volume II: Enzymolog Neil Osheroff,Mary-Ann Bjornsti Book 2001 Humana Press 2001

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Thokozile Manaka,Terence van Zyl,Deepak Kar complete double-helical turn). The end result of such a nicking-rejoining process in the presence of a DNA-unwinding agent is the formation of a relaxed underwound DNA molecule with a deficient linking number (the number of times one strand goes around the other in a duplex ring). The relaxed under

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Book 2001played an important role in the development and clinical application of antimicrobial and chemotherapeutic agents. The two volumes of DNA Topoisomerase Protocols are designed to help new and established researchers investigate all aspects of DNA topology and the function of these enzymes. The chapte

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Uncoupling of Topoisomerase-Mediated DNA Cleavage and Religation,nked to each of the generated 5′-ends. Several studies have utilized the detergent method to define the sites of interaction between DNA and topoisomerase I and II in vitro as well as in vivo (.,.). One of the major problems in these studies has been ascribed to the involvement of SDS in trapping cl

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