Titlebook: DNA Repair Protocols; Daryl S. Henderson Book 2006 Humana Press 2006 DNA.DNA replication.Ligation.PCR.Polyacrylamid-Gelelektrophorese.Quan

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Eric Allaire,Peter Libby,Alexander W. Clowesltures, tissue sections and biopsies, buccal cells, bone marrow aspirates, peripheral blood lymphocytes, and urine. Here we describe the use of a very sensitive RIA for the specific quantitation of cyclobutane dimers and (6-4) photoproducts in DNA extracted from mammalian cells and tissues.

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Complementation Assays Adapted for DNA Repair-Deficient Keratinocytes,s to UV irradiation of XP keratinocytes, and to objectively determine the extent to which cutaneous gene therapy may be realized, we set up experimental procedures adapted to ex vivo genetic complementation of keratinocytes from XP patients. We provide here detailed rationales and procedures for these approaches.

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Cytogenetic Challenge Assays for Assessment of DNA Repair Capacities,ociated with a significantly increased incidence of both cancer morbidity and mortality, the challenge assay may be useful in predicting cancer risk. The protocol for the assay is straightforward and the data have practical applications.

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Inhibition of DNA Synthesis by Ionizing Radiation,d MRE11, as well as downstream modulators can provoke an RDS phenotype. Here a simple, accurate and highly reproducible experimental protocol is presented for the generation of DNA synthesis inhibition curves from cells in culture.

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