Titlebook: DNA Repair; Methods and Protocol Lata Balakrishnan,Jason A. Stewart Book 2019 Springer Science+Business Media, LLC, part of Springer Nature

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審問,審訊

Kelly Comfort (Assistant Professor)-specific, defined SSB plasmid in . egg extracts. This experimental system can be applied in future studies to reveal many aspects of the molecular mechanisms of SSB repair and signaling in eukaryotes.

Carminative

https://doi.org/10.1007/978-3-031-14724-1ughput, solution-based equilibrium binding fluorescence polarization assay that can be applied to a wide array of protein–nucleic acid interactions. The assay is sensitive, stable, and able to identify small molecules capable of blocking DNA–protein interactions.

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Kelly Comfort (Assistant Professor)uorescent protein conversion system that can be conveniently used to measure the efficiency and analyze the lengths of conversion tracts of homology-directed genome editing using oligonucleotide donors in mammalian cells.

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https://doi.org/10.1057/978-1-349-95016-4ctivity using radiolabeled ATP. Additionally, we describe a high-throughput coupled reaction assay pairing ATP hydrolysis with nicotinamide adenine dinucleotide (NADH) oxidation to monitor ATP hydrolysis in real time.

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Thin-Layer Chromatography and Real-Time Coupled Assays to Measure ATP Hydrolysisctivity using radiolabeled ATP. Additionally, we describe a high-throughput coupled reaction assay pairing ATP hydrolysis with nicotinamide adenine dinucleotide (NADH) oxidation to monitor ATP hydrolysis in real time.

確定的事

Book 2019ve sections, each discussing a specific aspect of repair biology. Part I reviews the role of post-translational modifications of HDR, and DNA damage caused by defective telomere replication. Part II describes methods to detect and measure DNA breaks and proteins involved in DNA damage response. Part

吝嗇性

Homologous Recombination-Mediated DNA Repair and Implications for Clinical Treatment of Repair Defecng during DSB repair. In addition, clinical phenotypes associated with HR-defective cancers, such as mutational signatures used to predict response to poly(ADP-ribose) polymerase inhibitors, are discussed. Understanding these processes will provide insight into mechanisms of genome maintenance and l