Titlebook: DNA Recombination; Methods and Protocol Hideo Tsubouchi Book 2011 Springer Science+Business Media, LLC 2011 DNA double-strand break.DNA mic

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The Relevance of Beautiful Infrastructurewhich cells can use RNA to repair DNA damage such as a DSB are yet to be identified. Here we show the approach we utilize to detect repair of a chromosomal DSB by RNA-containing oligos in yeast cells.

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Methods to Study Mitotic Homologous Recombination and Genome Stabilityr a subset of the genomic instabilities: spontaneous mitotic recombination and chromosome loss, in both haploid and diploid cells. The assays, while not inclusive of all genome instability assays, give a broad assessment of general genome damage or inability to repair damage in various genetic backgrounds.

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Analysis of Meiotic Recombination Intermediates by Two-Dimensional Gel Electrophoresislso provide an introduction to meiotic time-course procedures, stabilization of branched DNA molecules by interstrand crosslinking, extraction of genomic DNA from meiotic cultures, and quantitative analysis of two-dimensional gel blots.

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Using the Semi-synthetic Epitope System to Identify Direct Substrates of the Meiosis-Specific Buddinlog, for kinase assays that detect only those proteins that are directly phosphorylated by Mek1. This method may be applicable to any kinase for which an analog-sensitive version is available. In addition, it provides a non-radioactive alternative for kinase assays with wild-type kinases.

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Detection of RNA-Templated Double-Strand Break Repair in Yeastwhich cells can use RNA to repair DNA damage such as a DSB are yet to be identified. Here we show the approach we utilize to detect repair of a chromosomal DSB by RNA-containing oligos in yeast cells.

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