Titlebook: DNA Nanotechnology; Methods and Protocol Giampaolo Zuccheri Book 2018Latest edition Springer Science+Business Media, LLC, part of Springer

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Giampaolo ZuccheriIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/260171.jpg

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The Synthesis of Designer DNA, here describes an enzymatic method for the synthesis of long repeat-sequence DNA. The method utilizes a PCR-based approach; starting with short oligo-seeds, c.a. 20?bp, bearing a minimum of two repeating units of >8?bp sequences. During each heat-cool cycle, the oligo-seeds reanneal imperfectly lea

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Preparation of Anti-miR PNAs for Drug Development and Nanomedicine,st-transcriptionally. PNAs are obtained with solid-phase peptide synthesis, and can be easily conjugated to other peptides. Conjugation with R8-Peptide or modification of the PNA backbone (at C5 or C2 carbon) with arginine side chains allows efficient cellular uptake. The present protocol describes

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Hybridization Chain Reaction Design and Biosensor Implementation,in impediments for such applications of DNA biosensors is their lack of sensitivity: this can prevent their full exploitation in the diagnostic analytical field. DNA nanotechnology could enhance DNA biosensors and let them perform at the required high sensitivity. Well-designed, programmable self-as

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Ultrasensitive Detection of Metal Ions with DNA Nanostructure,vy metal contamination remains challenging. Here we develop a DNA nanostructured microarray (DNM) with a tubular three-dimensional sensing surface and an ordered nanotopography for rapid and sensitive multiplex detection of heavy metal ions. In our design, DNA tetrahedral-structured probes (TSPs) ar