Titlebook: DNA Microarrays for Biomedical Research; Methods and Protocol Martin‘Dufva Book 20091st edition Humana Press 2009 Allele.DNA.Gene expressio

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On ,, , and , in Judicial Argumentations covering the chromosomal region of interest. Here we describe the methods developed at the Wellcome Trust Sanger Institute (Cambridge, UK) to construct microarrays comprising large-insert clones available through genome sequencing projects. These methods are applicable to Bacterial and Phage Artif

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https://doi.org/10.1007/978-94-007-0050-5The test and reference DNA samples are used as templates to generate two probe DNAs labeled with distinct fluorescent dyes. The two probe DNAs are co-hybridized on a microarray in the presence of Cot-1 DNA to suppress unspecific hybridization of repeat sequences. After slide washes and drying, micro

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Martin‘DufvaCovers the use of DNA array from the largest commercial providers.Includes practical details to design and produce your own probes and arrays.Provides methods for gene expression profiling, SNP mutati

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Comparative Genomic Hybridization: Microarray Design and Data Interpretation,r and constitutional DNA samples. This method has become increasingly flexible with the integration of new biological resources generated by genome sequencing projects. In this chapter, we describe alternative strategies for whole genome screening and high resolution breakpoint mapping of copy numbe

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Fabrication of DNA Microarray,rays, random bead arrays and suspension arrays. The different types of array can address different biological assays. Spotted arrays are suitable for application using small to medium number of probes, e.g. focused genotyping, bacterial diagnostics and gene expression analysis. In situ synthesized a