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Titlebook: DNA Damage and Repair in Human Tissues; Betsy M. Sutherland,Avril D. Woodhead Book 1990 Plenum Press, New York 1990 DNA.Laboratory.Xeroder

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書目名稱DNA Damage and Repair in Human Tissues
編輯Betsy M. Sutherland,Avril D. Woodhead
視頻videohttp://file.papertrans.cn/261/260140/260140.mp4
叢書名稱Basic Life Sciences
圖書封面Titlebook: DNA Damage and Repair in Human Tissues;  Betsy M. Sutherland,Avril D. Woodhead Book 1990 Plenum Press, New York 1990 DNA.Laboratory.Xeroder
描述Physical and chemical agents in the environment damage the DNA of humans, and pose a major threat to human health today, and to the genetic integrity of human populations. Although studies on isolated DNA in vitro, on prokaryotes, on mammalian cells in culture, and on laboratory animals have provided essential background information, it is now possible to study DNA damage and repair in human tissues directly. New techniques of high sensitivity, especially those not requiring radioactive labeling have made possible quantitation of DNA damage and repair, as well as detection of residual, unrepaired DNA lesions . In recent years, several investigators have taken up the challenge of studying damage and repair responses in humans, and we have chosen that work as the special focus of this Symposium. Major advances in under- standing damage and responses in human skin, in blood cells and in human internal organs indicate three major themes. First, DNA damage levels in human tissues depend not only on the initial exposures, but also on the capapacity of that tissue for repair of the specific lesion type. Second, repair in human tissues may differ quantitatively and qualitatively from that
出版日期Book 1990
關鍵詞DNA; Laboratory; Xeroderma pigmentosum; blood; blood cell; cancer; cells; chromosome; gene therapy; genes; pop
版次1
doihttps://doi.org/10.1007/978-1-4613-0637-5
isbn_softcover978-1-4612-7903-7
isbn_ebook978-1-4613-0637-5
copyrightPlenum Press, New York 1990
The information of publication is updating

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Lesion Measurement in Non-Radioactive DNA by Quantitative Gel Electrophoresisryotic cells growing in culture. The information obtainable from these model systems, while extremely valuable, can never replace the need for data from intact higher organisms. The gel electrophoresis method developed during the past ten years in our laboratories makes possible the quantitation of
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Fluorescence Detection of Lesions in DNA through diet, occupation and life-style (World Health Organization, 1973; 1983). Metabolism and activation of chemicals that reach target-organs lead to the formation of carcinogen-DNA adducts, which are recognized as human cancer risk factors (Doll and Peto, 1981)
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5-MOP Induced Protection Against Epidermal DNA Damage by Ultraviolet Radiation in Human Skinf skin types I and II who sunburn easily and have a limited ability to tan (MacKie et al., 1987). In the last few years this information has been widely publicized in the press and other media. Despite this, a suntan is still a prized social asset and epidemiological data indicate a continued rise i
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Variability in DNA Repair in Human Skinlation of rates of incidence with latitude and, presumbably, solar exposure (Scotto, 1986), occurence on sun-exposed body regions (Scotto, 1986) and higher incidence in individuals who spend more time outdoors (Urbach et al., 1972) point to the sun as a major etiologic agent. For melanoma, there is
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