Titlebook: DNA Cloning and Assembly; Methods and Protocol Sunil Chandran,Kevin W. George Book 2020 Springer Science+Business Media, LLC, part of Sprin

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1064-3745 ader, and tips on troubleshooting and avoiding known pitfalls...Authoritative and cutting-edge, .DNA Cloning and Assembly: Methods and Protocols .aims to ensure successful results in the further study of this vital field..978-1-0716-0910-1978-1-0716-0908-8Series ISSN 1064-3745 Series E-ISSN 1940-6029

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https://doi.org/10.1007/978-3-531-90438-2NGS libraries for pooled sequencing. This chapter describes a high-throughput protocol that enables the simultaneous sequencing of thousands of DNA constructs in a single sequencing run at a dramatically reduced cost compared to bench-top?methods.

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Computer — Mega-Maschine oder Werkzeug of execution. Here we describe a protocol for hierarchical assembly of large DNA constructs from modular DNA parts using the MetClo vector set, a set of assembly vectors designed for the MetClo method.

燒瓶

Highly Multiplexed, Semiautomated Nextera Next-Generation Sequencing (NGS) Library PreparationNGS libraries for pooled sequencing. This chapter describes a high-throughput protocol that enables the simultaneous sequencing of thousands of DNA constructs in a single sequencing run at a dramatically reduced cost compared to bench-top?methods.

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Hierarchical Modular DNA Assembly Using MetClo of execution. Here we describe a protocol for hierarchical assembly of large DNA constructs from modular DNA parts using the MetClo vector set, a set of assembly vectors designed for the MetClo method.

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https://doi.org/10.1007/978-3-658-36320-8he Standard European Vector Architecture and a flexible design that allows researchers to easily modify the vector backbone via secondary cloning sites. This flexibility allows for JUMP vectors to be used in a wide variety of applications and hosts.