Titlebook: DNA Arrays; Methods and Protocol Jang B. Rampal Book 2001 Humana Press 2001

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Formation of the Contract of Employmentrs of DNA samples (either complete cDNAs or ESTs) are immobilized as very high-density arrays on solid surfaces, typically glass. Microarray analysis is conceptually similar to that of a reverse dot blot, whereby the tethered “probe” DNA samples comprising the array are hybridized with fluorescently

ATRIA

https://doi.org/10.1007/978-94-017-6306-6nally developed by physicists and applied to biology is the microchip. The semiconductor industry manufactures silica chips with increasing numbers of smaller and smaller features, which allows incredible numbers of operations within split seconds. The same principle of performing multiparallel oper

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https://doi.org/10.1057/9781137551412 mRNA and inhibit translation. AONs show promise as therapeutics to many diseases caused by abnormal or unwanted expression of a gene, such as cancers. The first antisense oligonucleotide-based drug (Vitravene?: ISIS Pharmaceuticals) was introduced in 1998 to treat cytomegalovirus (CMV) retinitis in

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https://doi.org/10.1057/9780230376540sequencing of their DNA, unique opportunities are becoming available for studying genetic variation in humans and its relationship with disease risk and aging. Consequently, new techniques have been invented to rapidly screen genes for biological information.

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The Genesis of Arab Nationalismxt step is to scan in the glass, gel, or plastic slides with a specialized scanner to obtain digital images of the results of the experiment. The DNA expression levels are then quantified with the help of image-analysis software. After the image processing and analysis step is completed, we end up w

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The Genesis of Arab Nationalismies of two-dimensional confocal slices of the specimen at different focus positions. Additionally, confocal microscopes are excellent for measuring weak fluorescence from areas adjacent to areas of very strong fluorescence, because the confocal pinhole rejects light from areas outside the focus spot

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The Genesis of Arab Nationalismtionality, or both. Microfluidic circuitry has yielded the lab-on-a-chip concept in which functions such as sample processing, reagent combining, component separation, and detection all occur in sequence on microscale integrated devices. Highly parallel functionality manifests in DNA microarrays, wh