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Titlebook: cAMP Signaling; Methods and Protocol Manuela Zaccolo Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusi

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發(fā)表于 2025-3-21 17:26:17 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱cAMP Signaling
副標(biāo)題Methods and Protocol
編輯Manuela Zaccolo
視頻videohttp://file.papertrans.cn/243/242719/242719.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: cAMP Signaling; Methods and Protocol Manuela Zaccolo Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under exclusi
描述This volume discusses the latest techniques used by researchers to measure cAMP activity at the cell population, whole cell, and subcellular level. The techniques covered in the book address questions related to cAMP compartmentalization, which look at relevant protein-protein interactions that increase the spatial and temporal resolution of cAMP signals detection, and that can help in the integration of the increasingly complex information that is becoming available in this field. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Cutting-edge and comprehensive, .cAMP Signaling: Methods and Protocols, Second Edition. is a valuable resource for scientists and researchers who are interested in learning more about this important and developing field.?.Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com..
出版日期Book 2022Latest edition
關(guān)鍵詞mlCNBD-FRET reporters; endometrial cells; pancreatic beta cells; high-throughput real-time measurement;
版次2
doihttps://doi.org/10.1007/978-1-0716-2245-2
isbn_softcover978-1-0716-2247-6
isbn_ebook978-1-0716-2245-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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發(fā)表于 2025-3-21 22:39:30 | 只看該作者
Daniel Carvalho,Telmo Silva,Jorge Abreully performed in vitro using radio-labeled ATP. For in vivo studies, genetically encoded FRET-based sensors have become popular for monitoring PKA activity. Here, we show that it is also possible to apply such reporters in vitro. We describe how to express and purify milligram quantities of a FRET-b
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發(fā)表于 2025-3-22 07:40:05 | 只看該作者
Applications and Usability of Interactive TVgenetic methods utilize microbial rhodopsins to elicit neuronal de- or hyperpolarization. However, other optogenetic tools have emerged that allow influencing neuronal function by different approaches. In this chapter we describe the use of photoactivated adenylyl cyclases (PACs) as modulators of ne
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Applications and Usability of Interactive TVith PKA-repressed reporters to either carry out high throughput screens for small molecule inhibitors of these target enzymes or to assess hit compounds and their analogs from such screens. Here, we describe two methods for testing panels of such compounds. The first uses a growth assay for which gr
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發(fā)表于 2025-3-23 05:25:14 | 只看該作者
Carina Haro Granizo,Gonzalo Olmedoiomyocytes. However, as the differentiation process is lengthy and commercially available cells are expensive, the cell number is limited. Here we provide detailed information on how to scale down 2D cell cultures of hIPS-CMs for the purpose of cAMP FRET measurements, thereby extending the number of
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發(fā)表于 2025-3-23 06:46:20 | 只看該作者
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