Titlebook: cAMP Signaling; Methods and Protocol Manuela Zaccolo Book 2015 Springer Science+Business Media New York 2015 Adenosine 3′,5′-monophosphate.

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書目名稱cAMP Signaling影響因子(影響力)




書目名稱cAMP Signaling影響因子(影響力)學科排名




書目名稱cAMP Signaling網(wǎng)絡公開度




書目名稱cAMP Signaling網(wǎng)絡公開度學科排名




書目名稱cAMP Signaling被引頻次




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書目名稱cAMP Signaling年度引用




書目名稱cAMP Signaling年度引用學科排名




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書目名稱cAMP Signaling讀者反饋學科排名

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Recording Intracellular cAMP Levels with EPAC-Based FRET Sensors by Fluorescence Lifetime Imaging,wnstream cellular effectors. FRET-based sensors are ideal to visualize and measure these rapid changes of second messenger concentrations in time and place. Here, we describe the use of EPAC-based FRET sensors to measure cAMP with spatiotemporal resolution in cells by fluorescence lifetime imaging (

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A Novel Approach Combining Real-Time Imaging and the Patch-Clamp Technique to Calibrate FRET-Based cAMP) signaling and compartmentalization in living cells. These sensors allow estimation of relative changes of cAMP levels in real-time and intact cells. However, one of their major shortcomings is that they do not easily allow direct measurement of cAMP concentrations. This is mainly due to the fa

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Channel-Based Reporters for cAMP Detection,hat have been modified to transduce cAMP concentrations into electrical or fluorescent readouts that can be readily detected using patch clamp amplifiers, photomultiplier tubes, or cameras. Here we describe two complementary approaches for the detection and measurement of cAMP signals near the plasm

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Imaging Sub-plasma Membrane cAMP Dynamics with Fluorescent Translocation Reporters,rs, a large number of tools for cAMP measurements have been developed. While most cAMP reporters are designed to undergo changes in fluorescence resonance energy transfer (FRET), there are alternative techniques with advantages for certain applications. Here, we describe protocols for cAMP measureme

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