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Titlebook: Cytoskeleton Proteins; A Purification Manua Gerhard Isenberg Book 1995 Springer-Verlag Berlin Heidelberg 1995 Actin.Aktin.Microfilaments.Mi

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11#
發(fā)表于 2025-3-23 13:14:31 | 只看該作者
12#
發(fā)表于 2025-3-23 14:28:03 | 只看該作者
Peter Biro,Manfred Abel,Thomas PaschAmmonium sulfate precipitation is an appropriate method to fractionate proteins prior to purification by chromatographic procedures. It is also used for concentrating protein samples. Ultra-pure ammonium sulfate (e.g. supplied by Schwarz-Mann) is recommended. AS may be added as solid compound or by dilution from a saturated stock solution.
13#
發(fā)表于 2025-3-23 21:33:26 | 只看該作者
Peter Biro,Manfred Abel,Thomas PaschA 240 KDa filamin equivalent protein from Dictyostelium discoideum, which crosslinks and bundles actin filaments at the cell cortex.
14#
發(fā)表于 2025-3-24 01:33:22 | 只看該作者
An?sthesie bei seltenen ErkrankungenA 75 kDa spherical molecule of ~9 nm in diameter, probably dimeric (~150 kDa) in its native form. A heat unstable, non-calmodulin binding MAP of sea urchin eggs.
15#
發(fā)表于 2025-3-24 04:34:28 | 只看該作者
16#
發(fā)表于 2025-3-24 07:48:03 | 只看該作者
https://doi.org/10.1007/978-3-662-05712-4A multigene family of diverse 40–80 KDa type I (acidic) and type II (basic) polypeptides ranging from pI 4.8–8.0, constituating intermediate filaments (IF) in various epitelial cells and tissues.
17#
發(fā)表于 2025-3-24 10:50:53 | 只看該作者
Peter Biro,Manfred Abel,Thomas PaschA heterodimeric calmodulin binding protein from the erythrocyte membrane skeleton composed of α (103 kDa) and β (97 kDa) subunits, also present in vertebrate brain.
18#
發(fā)表于 2025-3-24 17:58:35 | 只看該作者
General Lab EquipmentThe following general equipment is required for routine cytoskeleton protein purifications:
19#
發(fā)表于 2025-3-24 20:04:34 | 只看該作者
Preparation of BuffersIn general, buffers are made up according to two methods:
20#
發(fā)表于 2025-3-25 03:01:16 | 只看該作者
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