| 書目名稱 | Cytoskeleton Dynamics | | 副標(biāo)題 | Methods and Protocol | | 編輯 | Helder Maiato | | 視頻video | http://file.papertrans.cn/243/242680/242680.mp4 | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 叢書名稱 | Methods in Molecular Biology | | 圖書封面 |  | | 描述 | .This volume details comprehensive state-of-the-art methods on actin microfilaments and microtubules and how they work to achieve different cellular functions in different cellular contexts. Chapters guide readers through protein purification, in vitro reconstitution of several cytoskeleton properties, analyses of microtubule- and actin-based structures, functional dissection of post-translational modifications, and roles in several biological processes. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...?Authoritative and cutting-edge, .Cytoskeleton Dynamics: Methods and Protocols .aims to provide a wide range of experimental approaches and be an invaluable resource for present and future generations of cytoskeleton researchers. .The chapter “Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes” is available open access under a Creative Commons Attribution 4.0 International License via l | | 出版日期 | Book 2020 | | 關(guān)鍵詞 | microtubules; Golgi-mediated microtubule organization; protein purification; in vitro reconstitution; cy | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-0716-0219-5 | | isbn_softcover | 978-1-0716-0221-8 | | isbn_ebook | 978-1-0716-0219-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2020 |
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