Titlebook: Cytochrome P450 Protocols; Ian R. Phillips,Elizabeth A. Shephard Book 2006Latest edition Humana Press 2006 Allele.Escherichia coli.Oxidati

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書目名稱Cytochrome P450 Protocols影響因子(影響力)




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書目名稱Cytochrome P450 Protocols網(wǎng)絡(luò)公開度學(xué)科排名




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書目名稱Cytochrome P450 Protocols被引頻次學(xué)科排名




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Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/242617.jpg

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Hans Peter Latscha,Helmut Alfons KleinA general procedure for the solubilization of cytochrome P450 (P450) from bacterial membranes specifically for a human P450 expressed heterologously in the host . co. is described. The example involves the use of a P450 (3A4) with a C-terminal oligohistidine tag and includes sequential DEAE and metal affinity chromatography.

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https://doi.org/10.1007/978-3-86226-287-8nes. The nomenclature is presented as a unifying principle to enhance communication across disciplines. Because of the historical nature of gene sequencing, sometimes names have to be changed, but this is kept to a bare minimum to avoid confusion in the literature. CYP names have now reached four di

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Hans Peter Latscha,Helmut Alfons Kleinbolizing enzymes toward a particular substrate. In this chapter we describe a baculovirus/insect cell system that we have used for the expression of human and mouse flavin-containing monooxygenases. Methods are described for the generation of recombinant baculoviral DNAs, via both site-specific tran

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Hans Peter Latscha,Helmut Alfons Kleinals. Individual P450 forms have unique, but overlapping, substrate specificities. It is necessary to determine which P450s play more important roles in the oxidation of these chemicals. A good way of studying the roles of P450s in the metabolism of these chemicals is to reconstitute the activities b