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Titlebook: Cyanidioschyzon merolae; A New Model Eukaryot Tsuneyoshi Kuroiwa,Shinya Miyagishima,Osami Misumi Book 2017 Springer Nature Singapore Pte Lt

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發(fā)表于 2025-3-26 21:33:56 | 只看該作者
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發(fā)表于 2025-3-27 10:08:55 | 只看該作者
Cellular Structure of ,: A Minimum Set of Organellesst (plastid), a simple-shaped ER, one Golgi body with two cisternae, a few vacuoles (lysosomes), and one peroxisome. During the last two decades, numerous electron and fluorescence microscopic studies, combined with synchronous culture, have demonstrated spatial organization and morphological inform
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發(fā)表于 2025-3-27 15:14:08 | 只看該作者
Procedures for Cultivation, Observation, and Conventional Experiments in ,e Collection at the National Institute of Environmental Studies in Japan. The strain has been cultured in 2× Allen’s medium or its derivatives. The optimal growth conditions for this strain are as follows: pH?2.5, 42?°C, and ~100?μmol photons m.?s., allowing the cell density to reach ~5?×?10. cells
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發(fā)表于 2025-3-28 08:08:26 | 只看該作者
Procedures for Transformation and Their Applications in ,ethods for nuclear gene targeting have been developed during the last decade. Currently, gene knockout, knock-in, and a stable expression of transgene are feasible in .. In addition, to examine the function of genes that are essential for cell viability, heat-shock inducible gene expression and nitr
40#
發(fā)表于 2025-3-28 11:22:28 | 只看該作者
Basic Techniques in Fluorescence and Electron Microscopyence and electron microscopy requires different procedures compared to those for green algae or higher plants. Here, fluorescence microscopy methods, such as cell staining with fluorescent dye and the setup of the immunofluorescence microscope, are described. For electron microscopy, methods for tra
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