Titlebook: Cryopreservation and Freeze-Drying Protocols; John G. Day,Michael W. Pennington Book 19951st edition Springer Science+Business Media New Y

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Ideology in the Act of Meaning,Deposition at cryogenic temperatures is generally considered to be the safest method for long-term storage of living materials. (For a general introduction into the storage of plant cell cultures, refer to Chapter 11.

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Cryopreservation and Freeze-Drying of Fungi,Many aspects of mycology today necessitate living cultures. Traditional taxonomy is being supplemented by molecular biological techniques often requiring growing organisms. The need to keep particular strains for confirmation of results, further studies, and as references is well established.

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Cryopreservation of Plant Protoplasts,Isolated plant protoplasts are most commonly liberated from tissues,

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Methods in Molecular Biologyhttp://image.papertrans.cn/d/image/240511.jpg

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Freeze-Drying and Cryopreservation of Bacteria,rovide varying degrees of success with different species of bacteria, and neither technique results in 100% recovery of preserved cells (.). There is no universally applicable method for the successful preservation of all bacteria, and where it is vitally important that cultures are not lost, it is advisable to use both methods in parallel.

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Freeze-Drying of Yeasts,on, under the terms of the Budapest Treaty (1977), has increased the demand for the maintenance of microorganisms in designated culture collections. As with other microorganisms, long-term preservation methods are required that ensure survival of the strain and the retention of any characteristics of commercial value.

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of suspended animation for extended periods. The removal of water from viable biological material in the frozen state (freeze-drying) provides another means of arresting the biological clock by withholding water, and commencing again by its addition.