Titlebook: Cryopreservation and Freeze-Drying Protocols; Willem F. Wolkers,Harri?tte Oldenhof Book 2015Latest edition Springer Science+Business Media

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Timothy Kerswell,Surendra Pratap to investigate crystallization, eutectic formation, glass transition, devitrification, recrystallization, melting, polymorphism, molecular relaxation, phase separation, water transport, thermochemistry, and kinetics of complex reactions (e.g., protein denaturation). Such information can be used for

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Timothy Kerswell,Surendra Prataping procedures for the cryopreservation of cells and tissue. Conventional cryomicroscopic assays, which rely on indirect evidence of intracellular freezing (e.g., opacity changes in the cell cytoplasm), can yield significant errors in the estimated kinetics. In contrast, the formation and growth of

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Timothy Kerswell,Surendra Prataplities including Raman spectroscopy, fluorescence correlation spectroscopy, fluorescence lifetime imaging, or multiphoton imaging. Multiphoton imaging can be used to study intracellular ice formation at the subcellular level. A Raman imaging modality can be used for chemical mapping of frozen sample

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Timothy Kerswell,Surendra Pratapts components through the use of low temperature as a physical fixation strategy” (Steinbrecht and Zierold, Cryotechniques in biological electron microscopy. Springer-Verlag, Berlin, p 293, 1987). The intention is to maintain confidence that the tissue observed retains the morphology and dimensions

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