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Titlebook: Cryo-Electron Tomography; Structural Biology i Friedrich F?rster,Ariane Briegel Book 2024 The Editor(s) (if applicable) and The Author(s),

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發(fā)表于 2025-3-21 17:34:16 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Cryo-Electron Tomography
副標題Structural Biology i
編輯Friedrich F?rster,Ariane Briegel
視頻videohttp://file.papertrans.cn/241/240489/240489.mp4
概述Comprehensive description of cryo-electron tomography.Addresses the underlying principles and all recent developments of cryo-ET.A valuable resource for scientists working in the field of structural b
叢書名稱Focus on Structural Biology
圖書封面Titlebook: Cryo-Electron Tomography; Structural Biology i Friedrich F?rster,Ariane Briegel Book 2024 The Editor(s) (if applicable) and The Author(s),
描述.This book presents key aspects and recent developments of cryogenic sample electron tomography (cryo-ET) methodology, authored by leading experts in the field. Understanding structure and function of biomolecules in the context of cells is a new frontier in cellular and structural biology. To facilitate such research, cryo-ET is a key method to visualize the molecules of life in their native settings. Cryo-ET enables the imaging of samples that are preserved in a near-native state, at (macro)-molecular resolution and in three dimensions. Thus, this technique is a unique tool to gain insights into how biomolecules?collaborate in orchestrating fundamental biological processes, how mutations cause diseases, pathogens cause infections, and to develop novel therapeutics to treat such illnesses.?This book provides a unique reference for the emerging field of cryo-ET.. The topics covered range from the fundamental principles of imaging to sample preparation, data analysis,and data sharing within the scientific community. It serves as a valuable resource for the next generation of structural biologists, making it suitable both for undergraduate students studying biochemistry, biophysics,
出版日期Book 2024
關鍵詞electron optics; phase contrast; tomography; tomographic reconstruction; Fourier-slice theorem; cryo-prep
版次1
doihttps://doi.org/10.1007/978-3-031-51171-4
isbn_softcover978-3-031-51173-8
isbn_ebook978-3-031-51171-4Series ISSN 1571-4853 Series E-ISSN 2542-9566
issn_series 1571-4853
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerl
The information of publication is updating

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Petra Lietz,Molie Tobin,Dita Nugroho. We then provide a comprehensive account of the current state-of-the-art cryo-ET?sample preparation techniques for specimens?ranging from in-vitro reconstitutions, single cells, to 3D cell cultures, and whole multicellular organisms. We point out specific considerations that facilitate a wide range
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Shauna Butterwick,Marilou Carrilloachine learning and advanced algorithms, and discuss challenges posed by the recent developments of deep learning methods. New approaches for localization of molecular complexes and spatial analysis are expected to provide precise molecular maps of diverse cellular compartments, elucidate the organi
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Telling Old Stories in New Waysg a wider context with equal detail. Electron tomography can also be performed on serial sections which allows entire cells or tissue volumes to be reconstructed at high resolution. Further, high precision correlative microscopy workflows can be implemented, permitting to combine information on mole
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Fundamentals of Instrumentation and Electron Optics for Cryo-Electron Tomography, eventually recorded in the image plane—by ever more sophisticated detectors. As we acknowledge that our sample fries all too quickly, we l also discuss low dose techniques and phase plates. Finally, we provide a short outlook towards exciting developments that may well push cryo-electron tomography
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