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Titlebook: Coronaviruses; Methods and Protocol Helena Jane Maier,Erica Bickerton,Paul Britton Book 2015 Springer Science+Business Media New York 2015

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發(fā)表于 2025-3-21 19:53:33 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Coronaviruses
副標(biāo)題Methods and Protocol
編輯Helena Jane Maier,Erica Bickerton,Paul Britton
視頻videohttp://file.papertrans.cn/239/238357/238357.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Coronaviruses; Methods and Protocol Helena Jane Maier,Erica Bickerton,Paul Britton Book 2015 Springer Science+Business Media New York 2015
描述.This volume aims to describe a variety of techniques that reflects the wide range of research currently performed in the field of coronavirology, and begins with an overview of current understandings of coronavirus replication and pathogenesis to introduce specialists and non-specialists to the field. The rest of the book is divided into several sections of chapters beginning with those that describe identification, diagnosis, and study of the evolution of coronaviruses. The next few chapters discuss the preparation of cells and organ cultures useful in propagating coronaviruses and titration techniques, as well as techniques for analyzing virus functions that require purification of the viruses. The next chapters describe two commonly used reverse genetics techniques for coronaviruses, and techniques detailing identification of cellular receptors, binding profiles of viral attachment proteins, and virus-cell fusion. The final chapters cover a broad spectrum of techniques to identify virus-host protein-protein interactions, confirm the functional role of these proteins in virus replication, study host cell responses through genome-wide or pathway-specific approaches, and visualize
出版日期Book 2015
關(guān)鍵詞coronovirology; titration techniques; viral protein; virus genomes; virus pathogenicity; virus replicatio
版次1
doihttps://doi.org/10.1007/978-1-4939-2438-7
isbn_softcover978-1-4939-4921-2
isbn_ebook978-1-4939-2438-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2015
The information of publication is updating

書目名稱Coronaviruses影響因子(影響力)




書目名稱Coronaviruses影響因子(影響力)學(xué)科排名




書目名稱Coronaviruses網(wǎng)絡(luò)公開度




書目名稱Coronaviruses網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Coronaviruses被引頻次




書目名稱Coronaviruses被引頻次學(xué)科排名




書目名稱Coronaviruses年度引用




書目名稱Coronaviruses年度引用學(xué)科排名




書目名稱Coronaviruses讀者反饋




書目名稱Coronaviruses讀者反饋學(xué)科排名




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Identification of a Novel Coronavirus from Guinea Fowl Using Metagenomicsent responsible for the fulminating disease of guinea fowl, we identified a novel avian gammacoronavirus associated with the disease using metagenomics. Next-generation sequencing is an unbiased approach that allows the sequencing of virtually all the genetic material present in a given sample.
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The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration of several respiratory viruses and as a viral assay system, using ciliostasis as the criterion for infection. A simple technique for the preparation of chicken tracheal organ cultures in glass test tubes, in which virus growth and ciliostasis can be readily observed, is described.
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Isolation and Propagation of Coronaviruses in Embryonated Eggso and its membranes provide the diversity of cell types that are needed for successful replication of a wide variety of different viruses. Within the family . the embryonated egg has been used as a host system primarily for two avian coronaviruses within the genus ., infectious bronchitis virus (IBV
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Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitihe IBV genome is inserted into the vaccinia virus genome under the control of a T7 promoter sequence. Vaccinia virus as a vector for the full-length IBV cDNA has the advantage that modifications can be introduced into the IBV cDNA using homologous recombination, a method frequently used to insert an
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