Titlebook: Confocal Microscopy; Methods and Protocol Stephen W. Paddock Book 19991st edition Humana Press 1999

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t advances have also occurred in the development of reagents and techniques for generating functional fluorescently tagged proteins. When these labeled proteins are used in conjunction with confocal and other advanced fluorescence microscopes, the dynamics of a given protein within the living cell c

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es. First, it would function in living animals, eliminating the need for fixation and dehydration and their associated artefacts. Second, it would permit each stage of development to be studied in a single, intact embryo. Third, it would function in all cell types and would reveal their morphology,

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lume regulation involves the removal of cells from the matrix and manipulation in culture (.). Measurements are often also needed from cells within intact tissue that are operating in their correct physiological context. These include the effects of cell-cell interactions and the mechanical, ionic,

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ence, and that do not strongly scatter or absorb either the excitation or emission light. This chapter provides protocols used to examine three such tissues: the cornea of the eye, the buccal mucosa (which lines the inner cheek), and the nasal respiratory epithelium. Although in each case our overal

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Confocal Microscopy978-1-59259-722-2Series ISSN 1064-3745 Series E-ISSN 1940-6029