Titlebook: Computational Methods in Synthetic Biology; Mario Andrea Marchisio Book 2021Latest edition Springer Science+Business Media, LLC, part of S

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finale

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https://doi.org/10.1007/978-1-4842-2586-8 provides efficient multiplex oligonucleotide design including specificity and primer dimer checks. Its fully automated workflow involves important design steps that use specific parameters to produce high-quality oligonucleotides. This chapter describes how these steps are computationally implemented by oli2go.

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Terminology and Key Mapping Conceptsnges. Detection and processing of multiple signals are crucial for many synthetic biology applications. A variety of logic circuits operating in living cells have been implemented. One particular class of logic circuits uses site-specific recombinases mediating specific DNA inversion or excision. Re

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resistant

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