Titlebook: Complement Methods and Protocols; B. Paul Morgan Book 2000 Humana Press 2000

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Screening for Complement Deficiency,less, because of the range of tests, complete testing may mean carrying out many different assays. In contrast, the determination of the prevalence of genetic complement deficiencies in populations requires a very few specific methods that are suitable for dealing with a large number of samples.

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Complement Methods and Protocols978-1-59259-056-8Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Purification of Complement Components, Regulators, and Receptors by Classical Methods,er species. Functional assays may, therefore, require modification. It should also be noted that antisera and monoclonal antibodies against a C component frequently do not crossreact with that component from other species.

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Immunoaffinity Methods for Purification of Complement Components and Regulators,ng the column to extremes of pH to disrupt antibody-antigen interaction. Some complement proteins are labile at these pH extremes and methods must be modified accordingly (.). Here, I will describe the steps followed in establishing an immunoaffinity protocol for a complement protein. These differ l