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Titlebook: Complement Methods and Protocols; B. Paul Morgan Book 2000 Humana Press 2000

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發(fā)表于 2025-3-21 19:53:38 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Complement Methods and Protocols
編輯B. Paul Morgan
視頻videohttp://file.papertrans.cn/232/231283/231283.mp4
概述Includes supplementary material:
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Complement Methods and Protocols;  B. Paul Morgan Book 2000 Humana Press 2000
描述The complement system, first described more than a century ago, was for many years the ugly duckling of the immunology world, but no more. Complement in recent years has blossomed into a fascinating and fast moving field of immediate relevance to clinical scientists in fields as diverse as transplantation biology, virology, and inflammation. Despite its emergence from the shadows, complement retains an unwarranted reputation for being “difficult.” This impression derives in large part from the superficially complicated nomenclature, a relic of the long and tortuous process of unraveling the system, of naming components in order of discovery rather than in a syst- atic manner. Once the barrier of nomenclature has been surmounted, then the true simplicity of the system becomes apparent. Complement comprises an activation system and a cytolytic system. The former has diverged to focus on complement to distinct targets—bacteria, - mune complexes, and others—so that texts now describe three activation pa- ways, closely related to one another, but each with some unique features. The cytolytic pathway is the same regardless of the activation process and kills cells by creating pores in th
出版日期Book 2000
版次1
doihttps://doi.org/10.1385/159259056X
isbn_softcover978-1-61737-116-5
isbn_ebook978-1-59259-056-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2000
The information of publication is updating

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發(fā)表于 2025-3-21 21:07:32 | 只看該作者
Purification of Complement Components, Regulators, and Receptors by Classical Methods,ilable in most biochemistry laboratories. None of these methods require the large amounts of specific antibodies needed for the popular and rapid immunoaffinity methods to be described in .. A further advantage of classical chromatography is that the methods described in this chapter for human C can
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發(fā)表于 2025-3-22 01:18:53 | 只看該作者
Immunoaffinity Methods for Purification of Complement Components and Regulators,rs, and regulators have been successfully isolated by immunoaffinity protocols. The use of these methods is limited only by the availability of suitable antibodies in sufficient quantities (.–.). Immunoaffinity methods are compatible with most detergents used in the solubilization of membrane protei
地板
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Immunochemical Measurement of Complement Components and Activation Products,ent proteolytic cleavage products, recruited from these inactive precursor molecules in a sequentially proceeding cascade (.). This leads to an alteration of their antigenic pattern and thus to the consumption of the nativemolecule. Concomitantly, complement activation initiates formation of multi-m
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發(fā)表于 2025-3-22 20:18:58 | 只看該作者
Complement Deposition in Tissues,and are detected by immunohistochemical methods. C3b, C4b, and their fragments remain covalently bound on targets and components of the membrane attack complex (MAC) can become inserted into cell membranes. In addition, many components (like C1q and properdin) are stable enough and have such a high
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發(fā)表于 2025-3-23 00:55:16 | 只看該作者
Complement Regulators and Receptors in Tissues,th appropriate antibodies. The bound antibodies are visualized with a proper label that has been attached to the primary antibody itself or to a secondary antibody against the primary immunoglobulin (..). Despite the label the antibody retains its reactivity with its antigen. In immunofluorescence m
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發(fā)表于 2025-3-23 04:44:20 | 只看該作者
Measurement of C3 Fragment Deposition on Cells, or regulation. However, other consequences of complement activation are probably of more relevance in vivo. Complement plays a major role in enhancing the generation of specific antibodies and provides an interface between the humoral and cellular immune responses (.–.). Complement also increases t
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發(fā)表于 2025-3-23 07:19:54 | 只看該作者
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