Titlebook: Colorectal Cancer; Methods and Protocol Jean-Fran?ois Beaulieu Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018

Dissonance

Classification of Colorectal Cancer in Molecular Subtypes by Immunohistochemistryin both clinical as well as biological properties. The gold-standard classification strategy relies on genome-wide expression data, which hampers widespread implementation. Here we describe an immunohistochemical (IHC) Mini Classifier, a practical tool that, in combination with microsatellite instab

Lineage

Stool DNA Integrity Method for Colorectal Cancer Detection PCR (RT PCR) analysis. FL-DNA has great potential in colorectal cancer (CRC) lesions detection used alone or in combination with the standard CRC screening tool: immunochemical fecal occult blood test (iFOBT).

arousal

RT-qPCR for Fecal Mature MicroRNA Quantification and Validation probe, real-time quantitative PCR analysis on exfoliated enriched colonocytes in stool, using human (., hsa) micro(mi)RNAs to monitor changes of their expression at various stages of colorectal (CRC) progression, this method allows for the reliable and quantitative diagnostic screening of colon can

MELON

A Stool Multitarget mRNA Assay for the Detection of Colorectal Neoplasmsroup has shown that mRNA stool assays represent a powerful and robust approach for the prediction of colorectal neoplasms. In this methodological chapter, we describe the procedures to isolate good quality stool RNA and the steps to evaluate the levels of specific host mRNA markers such as ITGA6, MY

PON

消息靈通

Proteomic Profiling for Colorectal Cancer Biomarker Discoveryitable techniques for searching new biomarkers. In this chapter, we describe methodology for biomarker discovery based on a proteomic approach. In addition, special attention is given to the sample preparation, including protein extraction, fractionation, and cleanup, as we consider this a critical

averse

核心

Methods for In Vivo Functional Studies of Chromatin-Modifying Enzymes in Early Steps of Colon Carcinng. In this chapter, we describe a chemically-mediated method to induce colorectal carcinogenesis that we have used to identify in vivo the role of two chromatin modifying enzymes belonging to the same multimolecular complex, the histone acetyltransferase Tip60 and the histone variant-incorporating ATPase p400.

overwrought

Proteomics Analysis of Colorectal Cancer Cellss and the necessary steps to obtain a specific proteomic profile of each cell line. This technique enables a comprehensive proteomic analysis of cancer cell lines and the identification of pathways that are deregulated in different cancer cell lines.

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