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Titlebook: Cohesin and Condensin; Methods and Protocol Kyoko Yokomori,Katsuhiko Shirahige Book 2017 Springer Science+Business Media New York 2017 cohe

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發(fā)表于 2025-3-21 18:30:37 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書(shū)目名稱(chēng)Cohesin and Condensin
副標(biāo)題Methods and Protocol
編輯Kyoko Yokomori,Katsuhiko Shirahige
視頻videohttp://file.papertrans.cn/230/229233/229233.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書(shū)名稱(chēng)Methods in Molecular Biology
圖書(shū)封面Titlebook: Cohesin and Condensin; Methods and Protocol Kyoko Yokomori,Katsuhiko Shirahige Book 2017 Springer Science+Business Media New York 2017 cohe
描述.The volume provides comprehensive, state-of-the-art experimental techniques that are now available to dissect the molecular mechanisms of regulation and function of cohesin and the related factor condensin in vitro and in vivo across different model organisms, as well as in human cells. .Cohesin and Condensin: Methods and Protocols .is divided into three parts: Part I explores various in vitro and in vivo systems used to study the fundamental mechanism of cohesin regulation in mitosis and meiosis; Part II summarizes experimental systems in a variety of organisms that are used to address interphase functions of cohesin and Nipbl in gene regulation and chromatin interaction, ribosome biogenesis and DNA repair, which contribute significantly to cohesion-associated disorders; Part III covers related condensin complex and describes techniques to study its role in mitosis and interphase. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutti
出版日期Book 2017
關(guān)鍵詞cohesin regulation; cell-free system in Xenopus; cohesin-dependent sister chromatid cohesion; loading f
版次1
doihttps://doi.org/10.1007/978-1-4939-6545-8
isbn_softcover978-1-4939-8232-5
isbn_ebook978-1-4939-6545-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2017
The information of publication is updating

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https://doi.org/10.1007/978-94-007-1324-6m chromatin is incubated with these extracts, it is first duplicated within an assembled nucleus, and is then transformed into mitotic chromosomes, in each of which sister chromatids are juxtaposed with each other in a cohesin-dependent manner. Here we describe our protocols for assembling duplicate
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Lecture Notes in Electrical Engineeringesion is terminated during the metaphase-to-anaphase transition to trigger sister-chromatid segregation. The establishment and dissolution of cohesion are highly regulated by the cohesin complex and its multitude of regulators. In particular, the cohesin regulator Wapl promotes the release of cohesi
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Lecture Notes in Electrical Engineeringposttranslational modifications, including phosphorylation, acetylation, ubiquitylation, and SUMOylation. Recent studies have shown that cohesin SUMOylation is essential for sister chromatid cohesion during normal cell cycle and in response to DNA damage. Posttranslational modification by the small
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A. Bulletti,L. Capineri,D. Floridiaque chromatin structures and chromatin associated protein complexes. The analysis of these structures and complexes has greatly benefited from and relied on the visualization of meiotic proteins in diverse preparations of meiocytes. In this chapter we summarize methods that can be used for the chara
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Sensors and Protocols for Industry 4.0P) to the internal red fluorescence protein (RFP) control. With this dual-color heterologous reporter assay, we have revealed cohesin-regulated genes and discovered a .-acting DNA element, the Ty1-LTR, which interacts with cohesin and regulates gene expression during yeast meiosis. The method descri
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