Titlebook: Clinical Applications of PCR; Rajyalakshmi Luthra,Rajesh R. Singh,Keyur P. Patel Book 2016Latest edition Springer Science+Business Media N

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Emulsion PCR: Techniques and Applications, is dilution and compartmentalization of template molecules in water droplets in a water-in-oil emulsion. Ideally, the dilution is to a degree where each droplet contains a single template molecule and functions as a micro-PCR reactor. Here, we discuss the basic principles, advantages, and challenge

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Quantitative PCR for Plasma Epstein-Barr Virus Loads in Cancer Diagnostics,Hodgkin’s lymphoma, Burkitt’s lymphoma, nasopharyngeal carcinoma, and HIV-related lymphomas. It infects nearly all humans and then persists for the life of the host in a small proportion of benign B lymphocytes. EBV reactivation, usually in the setting of immunosuppression, is the main risk factor f

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High-Resolution Melt Curve Analysis in Cancer Mutation Screen,f mutated versus wild-type DNA sequences. HRM analysis is a high-throughput, sensitive, and efficient alternative to Sanger sequencing and is used to assess for mutations in clinically important genes involved in cancer diagnosis. The technique involves PCR amplification of a target sequence in the

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Locked Nucleic Acid Probes (LNA) for Enhanced Detection of Low-Level, Clinically Significant Mutatiral high sensitivity methods are described, the incorporation of these new techniques in a clinical lab may be difficult if the technology is not readily available or requires major changes in the workflow of the laboratory. Techniques that are robust and easily adapted to existing laboratory protoc

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Genotyping of Frequent Mutations in Solid Tumors by PCR-Based Single-Base Extension and MassARRAY As has greatly impacted the clinical care of cancer patients based on the fact that many of these tumors exhibit activating mutations in driver genes that are prone to target therapy, largely impacting cancer management strategies. Genomic heterogeneity of tumors is becoming an increasingly recognize

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