Titlebook: Clathrin-Mediated Endocytosis; Methods and Protocol Laura E.‘Swan Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2

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Francis A. Gunther,Jane Davies Gunther during infection. Endocytosis activity is known to vary during the cell cycle, in particular during mitosis. Importantly, different experimental conditions can lead to opposite results and conclusions, thereby emphasizing the need for a careful design of protocols. For example, experiments using se

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https://doi.org/10.1007/978-1-4613-9388-7ncluding receptors for nutrients and signaling molecules, as well as synaptic vesicle reformation. Multiple genetic and chemical approaches have been developed to interfere with this process. However, many of these tools do not selectively block CME, for example by targeting components shared with c

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Gerald A. Pollock,Wendell W. Kilgorey not viable. Different approaches have been developed to circumvent this limitation, including resorting to mosaic model organisms. We here describe the use of FLP/FRT-mediated mitotic recombination to generate . having homozygous mutant eyes while the rest of their body is heterozygous. We then pr

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Soil-parathion surface interactions, recruitment of auxilin and Hsc70, an ATP-driven molecular clamp. Here, we describe the preparation of reagents and the single-particle fluorescence microscopy imaging assay in which we visualize directly the Hsc70-driven uncoating of synthetic clathrin coats or clathrin-coated vesicles.