Titlebook: Chromosome and Genomic Engineering in Plants; Methods and Protocol Minoru Murata Book 2016 Springer Science+Business Media New York 2016 pl

確定方向

Targeted Mutagenesis in Rice Using TALENs and the CRISPR/Cas9 System, occurs stochastically in transgenic cells and SSN-mediated targeted mutagenesis often leads to no selectable phenotype, identification of highly mutated cell lines is a critical step in obtaining regenerated plants with desired mutations.

concert

Development of Genome Engineering Tools from Plant-Specific PPR Proteins Using Animal Cultured Cell ratio (>80?%); and rapid, high-throughput data production. In this chapter, we introduce an example of such reporter systems: a PPR-based sequence-specific translational activation system. The cell-based reporter system can be applied to characterize plant genes of interested and to PPR engineering.

共同時代

Three-Dimensional, Live-Cell Imaging of Chromatin Dynamics in Plant Nuclei Using Chromatin Tagging escent signals within a nucleus and monitor the distance between homologous loci. These live-cell imaging methods will provide new insights into genome organization, development processes, and subnuclear responses to environmental stimuli in plants.

SEED

DAMP

FLAX

https://doi.org/10.1007/978-1-4020-6979-6Nihon bare” expressing . TPase. pDLHC has potential for the efficient generation of various types of chromosomal rearrangements including deletions, inversions, translocations and artificial ring chromosomes in plants, and the detailed protocol for rice is described here.

Insulin

Protein

RAG

One-Step Generation of Chromosomal Rearrangements in Rice,Nihon bare” expressing . TPase. pDLHC has potential for the efficient generation of various types of chromosomal rearrangements including deletions, inversions, translocations and artificial ring chromosomes in plants, and the detailed protocol for rice is described here.