Titlebook: Chromosome and Genomic Engineering in Plants; Methods and Protocol Minoru Murata Book 2016 Springer Science+Business Media New York 2016 pl

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Guillaume Cohen,Maxime Ladaiquespectively, allows enrichment of cells harboring target genes modified via gene targeting (GT). We have developed a successful GT system employing positive–negative selection and subsequent precise marker excision via the . transposon derived from the cabbage looper moth to introduce desired modific

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Guillaume Cohen,Maxime Ladaiquengineering of PPR motifs as new RNA/DNA manipulation tools in living cells, including for genome editing. However, the biochemical characteristics of PPR proteins remain unknown, mostly due to the instability and/or unfolding propensities of PPR proteins in heterologous expression systems such as ba

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The European Union Social Tools in Question repetitive DNA sequences. Despite the recent progress in the analysis of plant genome organization and chromosome structure, there is a need for easy methods to assign DNA sequences to individual chromosomes. Here, we describe an easy way to allocate genes or DNA sequences to chromosomes in wheat u

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Mary Ann Cooper,Ronald L. Holleresolution of FISH on chromosomes is limited by condensed structure of chromatin, and it is difficult to differentiate two target sites close to each other. To overcome this issue, the objects was changed to stretched DNA fibers, and this fiber FISH technique has now been used for revealing genome s

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