Titlebook: Chromatin Protocols; Srikumar P. Chellappan Book 2015Latest edition Springer Science+Business Media New York 2015 Chromatin imaging.Chroma

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Masaki Hata,Jaehyun Song,Yutaka Shinada is key for regulating gene expression and cell functions. The extent to which the original chromatin structure is altered after repair of DNA lesions is thus a critical issue. Dissecting histone dynamics at sites of DNA damage has provided mechanistic insights into chromatin plasticity in response

FRONT

https://doi.org/10.1007/978-1-4939-2474-5Chromatin imaging; Chromatin structure and function; DNA methylation; Histone modifications; Non-histone

GENRE

978-1-4939-4406-4Springer Science+Business Media New York 2015

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Preparation and Analysis of Positioned Mononucleosomes,r processes, including binding of proteins to nucleosomes, covalent histone modifications, transcription, DNA repair, and ATP-dependent chromatin remodeling. Here we describe several recently developed procedures for obtaining and analysis of mononucleosomes assembled on 200–350-bp DNA fragments.

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CANE

Springer Tracts in Modern Physicsr processes, including binding of proteins to nucleosomes, covalent histone modifications, transcription, DNA repair, and ATP-dependent chromatin remodeling. Here we describe several recently developed procedures for obtaining and analysis of mononucleosomes assembled on 200–350-bp DNA fragments.

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Preparation and Analysis of Positioned Mononucleosomes,r processes, including binding of proteins to nucleosomes, covalent histone modifications, transcription, DNA repair, and ATP-dependent chromatin remodeling. Here we describe several recently developed procedures for obtaining and analysis of mononucleosomes assembled on 200–350-bp DNA fragments.

ANTIC

Chromatin Imaging with Time-Lapse Atomic Force Microscopy,f high-speed AFM instrumentation made it possible to image the dynamics of proteins and protein-DNA complexes within millisecond time range. This chapter describes protocols for studies of structure and dynamics of nucleosomes with time-lapse AFM including the high-speed AFM instrument. The necessar

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Isolation of Specific Genomic Regions and Identification of Associated Molecules by Engineered DNA-inding molecule-mediated chromatin immunoprecipitation (enChIP) using the CRISPR system, for purification of specific genomic regions retaining molecular interactions. In this form of enChIP, specific genomic regions are immunoprecipitated with antibody against a tag(s), which is fused to a catalyti