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Titlebook: Chromatin Protocols; Peter B. Becker Book 19991st edition Springer Science+Business Media New York 1999

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Nucleosome Structure and Dynamics,cognized to result from DNA complexation with host histones to form minichromosomes only 10 years later (.). Because they are in contact with endogenous topoisomerases, minichromosomes are relaxed, that is, the linker DNAs that connect nucleosomes to one-another along the minichromosomes are free fr
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Analysis of Chromatin by Scanning Force Microscopy,icroscopical techniques with nucleosomal resolution (electron microscopy and the newly emerging probe microscopies) have been useful in understanding chromatin fiber structure. The most widely used probe microscopy for biological applications, the scanning force microscope (SFM), also known as the a
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In Vivo Mapping of Nucleosomes Using Psoralen-DNA Crosslinking and Primer Extension,ctural organization of the DNA for processes such as replication, transcription, recombination, and repair. The highly compact structure of chromatin does not only restrict the access of DNA to enzymes and regulating factors, it can even act as an activating principle bringing distant DNA-protein bi
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Preparation of Chromatin Assembly Extracts from , Oocytes, chromatin. Chromatin is a periodic structure made up of repeating, regularly spaced subunits, the nucleosomes. Elegant genetic experiments have clearly demonstrated that histones play a central role in transcriptional control (.). Moreover, histones, via protein-protein interactions or by playing a
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