Titlebook: Chromatin; Methods and Protocol Julia Horsfield,Judith Marsman Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive l

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Genome-Wide Mapping and Microscopy Visualization of Protein–DNA Interactions by pA-DamIDo this list with distinct advantages. pA-DamID relies on antibody-based targeting of the bacterial Dam enzyme, resulting in adenine methylation of DNA in contact with the protein of interest. This .A can then be visualized by microscopy, or mapped genome-wide. The main advantages of pA-DamID are an

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High-Resolution ATAC-Seq Analysis of Frozen Clinical TissuesAC-seq analysis of clinical specimens at a single-cell resolution reveals the cellular composition of the tissue contributing to the understanding of intra-tissue heterogeneity. Here we describe our method for nuclei isolation from frozen specimens with wide applicability across tissue types, produc

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Single-Molecule Multikilobase-Scale Profiling of Chromatin Accessibility Using m6A-SMAC-Seq and m6A-ymatic treatment. This property has been the basis of a number of sequencing-based assays for genome-wide identification and tracking the activity of CREs across different biological conditions, such as DNAse-seq, ATAC-seq, NOMeseq, and others. However, the fragmentation of DNA inherent to many of t

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Chromatin978-1-0716-2140-0Series ISSN 1064-3745 Series E-ISSN 1940-6029

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