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Titlebook: Chimeric RNA; Methods and Protocol Hui Li,Justin Elfman Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 Gene f

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發(fā)表于 2025-3-21 16:16:33 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Chimeric RNA
副標(biāo)題Methods and Protocol
編輯Hui Li,Justin Elfman
視頻videohttp://file.papertrans.cn/226/225311/225311.mp4
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Chimeric RNA; Methods and Protocol Hui Li,Justin Elfman Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 Gene f
描述This book provides numerous methods for identification, validation, and functional characterization of chimeric RNAs, herein described as any transcript which contains the nucleotide sequence of two distinct parental genes. Beginning with identification and characterization through bioinformatics tools, the book continues with experimental validation using RT-PCR, hybridization methods for RNA, and the use of mass spectrometry data for fusion protein validation, as well as functional and mechanistic studies of chimeric RNAs and translational applications. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Chimeric RNA: Methods and Protocols. is an ideal guide to this expansive field and an inspiration to researchers seeking to further our knowledge of these remarkable features of the transcriptome..
出版日期Book 2020
關(guān)鍵詞Gene fusion; Transcription-mediated fusions; Fusion transcripts; Bioinformatics; Translational applicati
版次1
doihttps://doi.org/10.1007/978-1-4939-9904-0
isbn_softcover978-1-4939-9906-4
isbn_ebook978-1-4939-9904-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2020
The information of publication is updating

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書目名稱Chimeric RNA網(wǎng)絡(luò)公開度學(xué)科排名




書目名稱Chimeric RNA被引頻次




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書目名稱Chimeric RNA讀者反饋學(xué)科排名




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Transportation of Radioactive Material,n of chimeric transcripts, especially when careful consideration is applied to experimental design. In this chapter, we provide guidelines and procedures for use of RT-PCR in detection and measurement of chimeric RNA expression.
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1064-3745 expertsThis book provides numerous methods for identification, validation, and functional characterization of chimeric RNAs, herein described as any transcript which contains the nucleotide sequence of two distinct parental genes. Beginning with identification and characterization through bioinform
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https://doi.org/10.1007/978-981-19-4518-2neral methodology used in processing and filtering of RNA-seq data, followed by filtering of multiple varieties of artifacts to nominate potentially relevant gene fusions. Functional relevance of gene fusions is assessed based on the predicted domain architecture of the putative fusion proteins.
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