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Titlebook: Chemosensitivity; Volume I: In Vitro A Rosalyn D. Blumenthal Book 2005 Humana Press 2005 cell.cell culture.cell death.chemotherapy.imaging.

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發(fā)表于 2025-3-21 17:41:37 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書(shū)目名稱Chemosensitivity
副標(biāo)題Volume I: In Vitro A
編輯Rosalyn D. Blumenthal
視頻videohttp://file.papertrans.cn/225/224936/224936.mp4
概述Includes supplementary material:
叢書(shū)名稱Methods in Molecular Medicine
圖書(shū)封面Titlebook: Chemosensitivity; Volume I: In Vitro A Rosalyn D. Blumenthal Book 2005 Humana Press 2005 cell.cell culture.cell death.chemotherapy.imaging.
描述A state-of-the art collection of readily reproducible laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Chemosensitivity, Volume 1: In Vitro Assays provides a panel of 16 in vitro measures of chemosensitivity in adherent and non-adherent cells for single agents and combinations of agents. In addition to immunohistochemical and imaging approaches, these assays include clonogenic, colorimetric, fluorometric, and physiological assays. Highlights include image analysis to assess drug sensitivity, high throughput approaches using green fluorescent protein, DIMSCAN (a microcomputer fluorescence-based assay), and the ChemoFx assay used in biotechnology. A companion volume, Volume 2: In Vivo Models, Imaging, and Molecular Regulators, provides protocols for classifying tumors into response categories and customizing chemotherapy regimens to individual patients.
出版日期Book 2005
關(guān)鍵詞cell; cell culture; cell death; chemotherapy; imaging; tumor
版次1
doihttps://doi.org/10.1385/1592598692
isbn_softcover978-1-61737-508-8
isbn_ebook978-1-59259-869-4Series ISSN 1543-1894 Series E-ISSN 1940-6037
issn_series 1543-1894
copyrightHumana Press 2005
The information of publication is updating

書(shū)目名稱Chemosensitivity影響因子(影響力)




書(shū)目名稱Chemosensitivity影響因子(影響力)學(xué)科排名




書(shū)目名稱Chemosensitivity網(wǎng)絡(luò)公開(kāi)度




書(shū)目名稱Chemosensitivity網(wǎng)絡(luò)公開(kāi)度學(xué)科排名




書(shū)目名稱Chemosensitivity被引頻次




書(shū)目名稱Chemosensitivity被引頻次學(xué)科排名




書(shū)目名稱Chemosensitivity年度引用




書(shū)目名稱Chemosensitivity年度引用學(xué)科排名




書(shū)目名稱Chemosensitivity讀者反饋




書(shū)目名稱Chemosensitivity讀者反饋學(xué)科排名




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發(fā)表于 2025-3-21 21:51:10 | 只看該作者
Clonogenic Cell Survival Assay,large colony or a clone. This cell is then said to be clonogenic. A cell survival curve is therefore defined as a relationship between the dose of the agent used to produce an insult and the fraction of cells retaining their ability to reproduce. Although clonogenic cell survival assays were initial
板凳
發(fā)表于 2025-3-22 00:30:27 | 只看該作者
High-Sensitivity Cytotoxicity Assays for Nonadherent Cells,ingle remaining viable cell. Two such assays are described here, which have been used with Raji B-lymphoma cells, and are applicable to other nonadherent target cells. The first is a cell-counting assay, performed over a 3-wk period, which provides a simple, reliable, and sensitive assay of cytotoxi
地板
發(fā)表于 2025-3-22 08:27:22 | 只看該作者
Sulforhodamine B Assay and Chemosensitivity,ug-screening applications. Its principle is based on the ability of the protein dye sulforhodamine B to bind electrostatically and pH dependent on protein basic amino acid residues of trichloroacetic acid-fixed cells. Under mild acidic conditions it binds to and under mild basic conditions it can be
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發(fā)表于 2025-3-22 12:09:08 | 只看該作者
Use of the Differential Staining Cytotoxicity Assay to Predict Chemosensitivity,ations have demonstrated the clinical value of chemosensitivity testing with that assay (and similar ones). The DiSC assay is successful in the majority of malignant samples of which the cells can be brought in suspension (not necessarily as single-cell suspension). Although the assay is laborious a
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發(fā)表于 2025-3-22 16:11:05 | 只看該作者
Collagen Gel Droplet Culture Method to Examine In Vitro Chemosensitivity,ancer. We have developed a new in vitro chemosensitivity test named the collagen gel droplet embedded culture drug sensitivity test (CD-DST). The CD-DST method consists of a collagen gel droplet embedded culture step, exposure and washout of anticancer drug, a serum-free culture step, and evaluation
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發(fā)表于 2025-3-22 19:54:22 | 只看該作者
The MTT Assay to Evaluate Chemosensitivity,r cytokines on both normal and malignant cell populations. This can be accomplished by either direct or indirect counting methods. Direct counting by manual or automated methods, using a hemacytometer or particle counter, respectively, allows for serial cell counting at multiple time points, but the
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發(fā)表于 2025-3-22 22:48:04 | 只看該作者
Histoculture Drug Response Assay to Monitor Chemoresponse,lium bromide (MTT) end point among several modified HDRA procedures. Fresh surgical specimens are cut into approx 1- to 2-mm. pieces and put on a gelatin sponge infiltrated with culture medium containing a test drug. After incubation for 7 d, cell viability is assessed by the MTT assay. HDRA uses ca
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