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Titlebook: Chemical Protein Synthesis; Xuechen Li Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Scie

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51#
發(fā)表于 2025-3-30 08:50:48 | 只看該作者
https://doi.org/10.1007/978-1-4302-0310-0led procedures for the synthesis of Fmoc-protected SetCys residue and for its incorporation into peptides using standard solid-phase peptide synthesis protocols. We also describe its use for the chemical synthesis of proteins through the redox-controlled assembly of three peptide segments in one-pot.
52#
發(fā)表于 2025-3-30 12:24:42 | 只看該作者
53#
發(fā)表于 2025-3-30 18:24:25 | 只看該作者
54#
發(fā)表于 2025-3-30 21:20:26 | 只看該作者
55#
發(fā)表于 2025-3-31 03:51:27 | 只看該作者
56#
發(fā)表于 2025-3-31 08:06:54 | 只看該作者
Continuous-Flow Synthesis of ,-Methylated Peptides via Generation of an Acyl ,-Methylimidazolium Caent amounts of amino acids. The addition of a strong Br?nsted acid is critical to generate the highly reactive .-methylimidazolium cation species to accelerate the amidation. The developed approach enabled the synthesis of a bulky peptide with a higher yield in a shorter amount of time compared with the results of conventional amidation.
57#
發(fā)表于 2025-3-31 11:11:31 | 只看該作者
,Covalent Reactive Peptides to Block Protein–Protein Interactions and Inhibit Microbe–Host Interactihost cells instead of bacterium cells. Here we present the experimental details of the design and synthesis of cysteine-reactive peptides to selectively block Nck-SH3.2 but not the other two SH3 domains. Procedures of EPEC infection, covalent reaction inside Caco-2 cells, and bacterial counting to check the antibacterial effect are also described.
58#
發(fā)表于 2025-3-31 16:42:49 | 只看該作者
59#
發(fā)表于 2025-3-31 18:04:10 | 只看該作者
Side-Chain Anchoring Strategies for the Synthesis of Peptide Thioesters and Selenoesters,y. Importantly, this methodology overcomes solubility issues and epimerization of the C-terminal amino acid residue that can occur using solution-phase approaches. Detailed methods for the solid-phase synthesis of peptide thioesters and selenoesters using a side-chain anchoring approach are outlined in this article.
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