Titlebook: Characterization of Nanoparticles Intended for Drug Delivery; Scott E. McNeil Book 2018Latest edition Springer Science+Business Media LLC

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Updated Method for In Vitro Analysis of Nanoparticle Hemolytic Propertieshemolysis. The revision includes updated details about nanoparticle sample preparation, selection of nanoparticle concentration for the in vitro study, updated details about assay controls and case studies about nanoparticle interference with the in vitro hemolysis assay.

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In Vitro Assessment of Nanoparticle Effects on Blood CoagulationIC). In contrast, unintended inhibition of the coagulation pathways may lead to hemorrhage. Thrombogenicity is the property of a test material to induce blood coagulation by affecting one or more elements of the clotting process. Anticoagulant activity refers to the property of a test material to in

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Assessing NLRP3 Inflammasome Activation by Nanoparticlessimilar immune responses for intravenously injected, drug-containing nanoparticles can result in immunotoxicity (e.g., silica nanoparticles). Evaluation of NLRP3-mediated inflammasome activation by nanoparticles may predict pro-inflammatory responses in order to determine if these effects may be mit

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Methods for Analysis of Nanoparticle Immunosuppressive Properties In Vitro and In Vivoaterial. The antigen-specific leukocyte proliferation is then measured by ELISA analyzing incorporation of BrdU into DNA of the proliferating cells. Here, we describe the experimental procedures for investigating immunosuppressive properties of nanoparticles by both TDAR and HuLa assays, discuss the

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Analysis of Pro-inflammatory Cytokine and Type II Interferon Induction by Nanoparticles incubated with controls and tested nanomaterials for 24?h. Then, culture supernatants are analyzed by ELISA to detect IL-1β, TNFα, IL-8, and IFNγ. The culture supernatants can also be analyzed for the presence of other biomarkers secreted by the immune cells. Such testing would require additional a

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Scott E. McNeilIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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https://doi.org/10.1007/978-1-4939-7352-1nanotechnology; nanomaterials; nanomedicine; drug release; sterility and endotoxin; physicochemical; intra

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