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Titlebook: Chaperones; Methods and Protocol Stuart K. Calderwood,Thomas L. Prince Book 2023Latest edition The Editor(s) (if applicable) and The Author

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Targeted Replacement of HSF1 Phosphorylation Sites at S303/S307 with Alanine Residues in Mice Incre mouse model indicated the susceptibility of the mutant mice to become obese with age due to an increase in basal levels of heat shock proteins (HSPs) and chronic inflammation. Since HSF1 transcriptional activity is increased in many tumor types, this mouse model may be a useful tool for studies related to cellular transformation and cancer.
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Complementation Assays for Co-chaperone Function,functionality of eukaryotic JDPs can be assessed in a thermosensitive . mutant strain of the yeast . (JJ160). Here we outline the use of these thermosensitive microorganisms in complementation assays to functionally characterize a JDP from the bacterium, . (AgtDnaJ), and a JDP from the trypanosomal parasite, . (TcJ2).
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Optimized Microscale Protein Aggregation Suppression Assay: A Method for Evaluating the Holdase Actnce-based miniaturized versions of the standard aggregation suppression assay that are affordable and have wide application for low abundance holdases. The assay can be used for both fundamental characterization of holdase function in proteins and screening of inhibitors of holdase activity.
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