Titlebook: Cellular Quiescence; Methods and Protocol H. Daniel Lacorazza Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018

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Flow Cytometric Detection of G0 in Live Cells by Hoechst 33342 and Pyronin Y Staining,ve the same amount of DNA as cells at G1 phase but lower RNA levels compared to proliferating cells. Therefore, resting cells in G0 phase can be distinguished from proliferating cells in G1, S, and G2?M phases. This chapter describes a protocol for double staining of live cells with Hoechst 33342 an

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Isolation of Neural Stem and Progenitor Cells from the Adult Brain and Live Imaging of Their Cell Conal precursors in the adult mammalian brain. Various approaches of fluorescent-activated cell sorting (FACS) have emerged to allow the distinction between quiescent NSCs (qNSCs), their activated counterpart (aNSCs), and the resulting progeny. In this article, we review two FACS techniques that can