Titlebook: Cellular Imaging; Electron Tomography Eric Hanssen Book 2018 Springer International Publishing AG 2018 3D Reconstruction of Images.Analysi

鍵琴

荒唐

1618-7210 transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation.978-3-319-88699-2978-3-319-68997-5Series ISSN 1618-7210 Series E-ISSN 2197-5647

楓樹

Organisationsentwicklung und Fachlichkeito reconstruct 3-D images of the specimen in near-native state to ‘macromolecular’ resolution. In this introductory chapter we take the perspective of the workflow of a cryo-tomography project to outline the technique, and important underlying concepts.

DEFT

JOG

Innovation und Besch?ftigungsstabilit?t at a dose low enough to avoid radiation damage, yielding noisy tomograms with missing information. The quality of the tomograms depends on the algorithmic details, but also on the pre-processing of the tilt series images, and post-processing of the tomographic volume.

角斗士

CANE

https://doi.org/10.1007/978-3-531-90943-1with relative ease, we expect the use of STEM tomography to increase. The concepts for STEM imaging are significantly different than for TEM, and therefore we will describe in detail the STEM imaging modality, followed by STEM tomography concepts and applications.

善于

單調性

BOOM

,Conjugate Immunofluorescence—SEM Array Tomography for Studying Mammalian Synapses and Axons,owed by computational volume reconstruction and analysis. Conjugate immunofluorescence—SEM array tomography has been used for the study of brain tissue, and in particular for the characterization of diverse synapses and axons.