Titlebook: Cellular Heterogeneity; Methods and Protocol Natasha S. Barteneva,Ivan A. Vorobjev Book 2018 Springer Science+Business Media, LLC 2018 Live

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Quantification of the Metabolic Heterogeneity in Mycobacterial Cells Through the Measurement of the gy of Mtb, which may contribute to the variable response of intracellular Mtb to different antimycobacterial agents. In this chapter, we describe the details of a method used in the generation of reporter strains for the measurement of the NADH/NAD. ratio in mycobacteria. Importantly, once the repor

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Image-Based Tracking of Heterogeneous Single-Cell Phenotypess are beginning to capture the importance and interdependence of these phenomena. However, existing image analysis pipelines often fail to capture the intricate changes that occur in small subpopulations, either due to poor segmentation protocols or cell tracking errors. Here we report a pipeline de

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Broad Immune Monitoring and Profiling of T Cell Subsets with Mass Cytometryents of protein or nucleic acid abundances by bringing together the detection capacity of atomic mass spectroscopy and the sample preparation workflow typical of regular flow cytometry. In 2014 the mass cytometer was adapted for the acquisition of samples from microscopy slides (termed imaging mass

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Multiparametric Analysis of Myeloid Populations by Flow Cytometryusing fluorescently conjugated antibodies for specific surface and activation markers provides a comprehensive profile of immune cells. This chapter describes the identification and characterization of myeloid populations using flow cytometric analysis in an acute model of resolving inflammation. Th

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