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Titlebook: Cell-Cycle Synchronization; Methods and Protocol Zhixiang Wang Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive l

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發(fā)表于 2025-3-21 19:17:19 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書(shū)目名稱Cell-Cycle Synchronization
副標(biāo)題Methods and Protocol
編輯Zhixiang Wang
視頻videohttp://file.papertrans.cn/223/222941/222941.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書(shū)名稱Methods in Molecular Biology
圖書(shū)封面Titlebook: Cell-Cycle Synchronization; Methods and Protocol Zhixiang Wang Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive l
描述.This volume covers a broad range of cell types including cultured cell lines, primary cells, and various unicellular organisms such as fission yeast, budding yeast, parasite .Leishmania amazonensis, .and parasite .Trypanosoma brucei. .The chapters in this book are organized into four parts. Part One looks at a general overview of cell cycle control and synchronization. Part Two discusses techniques to synchronize mammalian cells to various cell cycle phases including mitotic sub-phases. Part Three covers synchronization of unicellular organisms and Part Four analyzes cell cycle progression. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. . Cutting-edge and thorough, .Cell-Cycle Synchronization: Methods and Protocols. is a valuable resource for both novice and expert scientists in this developing field. .
出版日期Book 2022
關(guān)鍵詞Drosophila; Xenopus; Flow cytometry; immunofluorescence; flowcytometry
版次1
doihttps://doi.org/10.1007/978-1-0716-2736-5
isbn_softcover978-1-0716-2738-9
isbn_ebook978-1-0716-2736-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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沙發(fā)
發(fā)表于 2025-3-21 21:16:41 | 只看該作者
Jo?o Fontes da Costa,Teresa Carla Oliveira cycle phase markers, and new technologies, cell cycle analysis continues to be a dynamic field within the flow cytometry community. This chapter will give an overview of the current state of cell cycle analysis by flow cytometry.
板凳
發(fā)表于 2025-3-22 01:35:15 | 只看該作者
Flow Cytometry and Cell Cycle Analysis: An Overview cycle phase markers, and new technologies, cell cycle analysis continues to be a dynamic field within the flow cytometry community. This chapter will give an overview of the current state of cell cycle analysis by flow cytometry.
地板
發(fā)表于 2025-3-22 06:42:36 | 只看該作者
5#
發(fā)表于 2025-3-22 10:55:02 | 只看該作者
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發(fā)表于 2025-3-22 15:51:32 | 只看該作者
Synchronization of , Cell Cycle Using Hydroxyureahronization. Here, we described a useful method to synchronize procyclic promastigote forms of . using hydroxyurea (HU) and the analysis of its DNA content profile. This approach can be extended to other trypanosomatids, such as . or ., and provides an effective method for arresting more than 80% of cells at the G1/S phase transition.
7#
發(fā)表于 2025-3-22 17:20:08 | 只看該作者
Synchronization of , by Counter-Flow Centrifugal Elutriationoliferate without lag, allowing subsequent cell cycle phases to be obtained by continued culture. The synchronized cell cultures obtained by this process have uniform DNA content, a narrow size distribution, undergo synchronous division, and maintain synchrony into subsequent cell cycles.
8#
發(fā)表于 2025-3-23 00:32:20 | 只看該作者
Marybeth Gasman,Felecia Commodored combining thymidine block with MG132 to synchronize cells in mitosis. These inhibitors are reversible and mitotic cells can be released into the G1 phase synchronously. These techniques can be applied to both Western blot and timelapse imaging to study mitotic progression.
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發(fā)表于 2025-3-23 01:43:17 | 只看該作者
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發(fā)表于 2025-3-23 06:02:35 | 只看該作者
Optimizing Supply Chain Performanceoliferate without lag, allowing subsequent cell cycle phases to be obtained by continued culture. The synchronized cell cultures obtained by this process have uniform DNA content, a narrow size distribution, undergo synchronous division, and maintain synchrony into subsequent cell cycles.
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