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Titlebook: Cell-Based Microarrays; Methods and Protocol Peter Ertl,Mario Rothbauer Book 2018 Springer Science+Business Media, LLC, part of Springer Na

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發(fā)表于 2025-3-21 19:28:30 | 只看該作者 |倒序?yàn)g覽 |閱讀模式
書目名稱Cell-Based Microarrays
副標(biāo)題Methods and Protocol
編輯Peter Ertl,Mario Rothbauer
視頻videohttp://file.papertrans.cn/223/222933/222933.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Cell-Based Microarrays; Methods and Protocol Peter Ertl,Mario Rothbauer Book 2018 Springer Science+Business Media, LLC, part of Springer Na
描述.This volume provides a comprehensive look at the current fabrication methods, materials, cell patterning techniques, and practical application of live-cell microarrays. The chapters in this book are categorized into six parts: introduction, biointerface engineering and live-cell microarrays, printing techniques for live-cell microarrays, live-cell microarray application, microfluidic live-cell arrays, and advanced materials suitable for live-cell microarrays. Some of the topics covered are artificial micro-niche array with spatially structured biochemical cues; inkjet printing for biomedical application; fabrication of multi-electrode arrays for neurobiology applications; rapid prototyping of thermoplastic microfluidic devices; and oxygen-generating photocrosslinkable hydrogel. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. . Cutting-edge and thorough, .Cell-Based Microarrays: Methods and Protocols. is a valuable resource for anyone
出版日期Book 2018
關(guān)鍵詞3D cell models; hydrogel; nanodroplet; micropatterning; antibody
版次1
doihttps://doi.org/10.1007/978-1-4939-7792-5
isbn_softcover978-1-4939-9286-7
isbn_ebook978-1-4939-7792-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2018
The information of publication is updating

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發(fā)表于 2025-3-22 00:01:08 | 只看該作者
Beyond the Ideal Political Apologyive patterning of biomolecules on thiol–ene solid supports. We describe the preparation of solid supports which are required for the immobilization, including porous monoliths, as well as two different immobilization schemes based on biotin–streptavidin interactions and covalent linkage via free amino groups respectively.
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Three-Dimensional Cell-Based Microarrays: Printing Pluripotent Stem Cells into 3D Microenvironmentsribe the methodology for production of alginate- and Matrigel-based 3-D cell microarrays for the study of mouse and human pluripotent stem cells on two different chip-based platforms. We further provide protocols for on-chip proliferation/viability analysis and the assessment of protein expression by immunofluorescence.
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發(fā)表于 2025-3-22 11:55:34 | 只看該作者
Fabrication of Biomolecule Microarrays for Cell Immobilization Using Automated Microcontact Printingtomation process. Here, we describe the production of biomolecule microarrays composed of extracellular matrix protein for the fabrication of cell microarrays by using an automated microcontact printing device. Large scale cell microarrays can be reproducibly obtained by this method.
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發(fā)表于 2025-3-22 14:51:26 | 只看該作者
Hydrogel Printing Based on UV-Induced Projection for Cell-Based Microarray Fabricationad of a physical mask, which also offers a high degree of flexibility and repeatability. Furthermore, the ability of PEGDA film to hinder cell adhesion makes it possible to control area over which cells attach.
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發(fā)表于 2025-3-22 19:43:19 | 只看該作者
https://doi.org/10.1057/9781137339652ribe the methodology for production of alginate- and Matrigel-based 3-D cell microarrays for the study of mouse and human pluripotent stem cells on two different chip-based platforms. We further provide protocols for on-chip proliferation/viability analysis and the assessment of protein expression by immunofluorescence.
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發(fā)表于 2025-3-22 23:11:58 | 只看該作者
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發(fā)表于 2025-3-23 04:14:41 | 只看該作者
Julie Cruikshank,Tatiana Argounova-Lowad of a physical mask, which also offers a high degree of flexibility and repeatability. Furthermore, the ability of PEGDA film to hinder cell adhesion makes it possible to control area over which cells attach.
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發(fā)表于 2025-3-23 08:36:37 | 只看該作者
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