Titlebook: Cell Viability Assays; Methods and Protocol Oliver Friedrich,Daniel F. Gilbert Book 2023Latest edition The Editor(s) (if applicable) and Th

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Oliver Friedrich,Daniel F. GilbertIncludes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/222896.jpg

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Average Rheological Quantities of Cells in Monolayersgether in a single layer. Here we describe step-by-step procedure as to how to employ a modified commercial rotational rheometer to run rheological measurement and detect average viscoelastic properties of cells while maintaining the necessary precision level at the same time.

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Assaying Proliferation Characteristics of Cells Cultured Under Static Versus Periodic Conditionsf cellular growth under static versus pulsed-perfused conditions, mimicking continuous replacement of extracellular fluid in the physiological environment. The protocol involves long-term life-cell high-content time-lapse imaging of fluorescent cells at 37?°C and ambient CO. concentration using mult

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Network Reconstruction as a Novel High-Level Marker of Functional Neuronal Viabilityas the modularity, the centrality, or the characteristic path length. In summary, these parameters describe the network and how it is influenced by experimental modulations, for example, hypoxia, nutrient deficiency, co-culture models, or application of drugs and other factors.

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Assessment of Cell Viability in Electrically Excitable Muscle Cells Through Intact Twitch Stimulatio this chapter, we describe step-by-step protocols to (i) obtain intact single muscle fibers from freshly dissected muscle tissue using an enzymatic digestion procedure and (ii) provide a workflow for the assessment of twitch response of single fibers that can be ultimately classified as viable. For

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Ordinary and Partial Differential Equationsf cellular growth under static versus pulsed-perfused conditions, mimicking continuous replacement of extracellular fluid in the physiological environment. The protocol involves long-term life-cell high-content time-lapse imaging of fluorescent cells at 37?°C and ambient CO. concentration using mult

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