Titlebook: Cell Surface Receptors: A Short Course on Theory and Methods; A Short Course on Th Lee E. Limbird Book 1996Latest edition Kluwer Academic P

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The Most Outstanding Ontologies,tes for these radiolabeled agents in target tissues where they elicit a physiological effect. This chapter will describe methods for the identification of radioligand binding sites and for obtaining data to establish whether the observed binding sites represent the physiological “receptor” for a particular hormone, neurotransmitter, or drug.

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Historical Perspective, historical account of the development of receptor theory in this chapter, as well as the outline of how to characterize receptors in intact tissues in chapter 2, will emphasize early investigations of drug action rather than (for example) physiological studies of hormone action. However, the reader

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Methods for Characterization of Receptors Based on Receptor-Mediated Responses in Tissue or Intact upancy and the ultimate physiological response. The present chapter (1) summarizes some methods used to characterize the specificity of the receptor that elicits an observed response, and (2) outlines methods for determining the affinity constants of agonist, partial agonist, and antagonist agents.

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Identification of Receptors Using Direct Radioligand Binding Techniques,tes for these radiolabeled agents in target tissues where they elicit a physiological effect. This chapter will describe methods for the identification of radioligand binding sites and for obtaining data to establish whether the observed binding sites represent the physiological “receptor” for a par

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Complex Binding Phenomena, linear transformations of binding data were derived assuming that a reversible bimolecular reaction occurred between ligand and receptor and that this interaction obeyed mass action law, namely .. + . ? ... Consequently, when data transformations such as the Scatchard plot are nonlinear, when Hill

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