Titlebook: Cell Cycle Control and Dysregulation Protocols; Antonio Giordano,Gaetano Romano Book 2004 Humana Press 2004

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Antonio Giordano,Gaetano RomanoIncludes supplementary material:

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The Essence of Acts of Empathy,f nuclear architecture and cytoarchitecture. High-resolution . immunofluorescence approaches permit assessment of functional interrelationships between nuclear structure and gene expression that are linked to the intranuclear compartmentalization of nucleic acids and regulatory proteins (an example

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The Essence of Acts of Empathy,ine). Nonradioactive ribonucleic acid (RNA) precursors (e.g., 5-bromouridine-5′-triphosphate [BrUTP]) are used and can be detected by using fluorescently labeled antibodies. Procedures for BrUTP of labeling transcription sites require manipulations that are best applied to adherent cells but can be

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https://doi.org/10.1007/978-94-017-6063-8and green fluorescent protein (GFP). Both molecules pose limitations with in vivo detection. When using GFP, autofluorescence may be encountered in tissues and tissue sections. Similarly, when using β-gal as a marker, the penetration of X-gal substrate is difficult in tissues with extensive extra ce