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Titlebook: Cell Culture Techniques; Michael Aschner,Lucio Costa Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer Nature

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發(fā)表于 2025-3-21 17:53:13 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Cell Culture Techniques
編輯Michael Aschner,Lucio Costa
視頻videohttp://file.papertrans.cn/223/222773/222773.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
叢書名稱Neuromethods
圖書封面Titlebook: Cell Culture Techniques;  Michael Aschner,Lucio Costa Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer Nature
描述This volume discusses the requirements, advantages, and limitations of studying cell culture. The chapters in this book cover topics such as in vitro blood-brain barrier functional assays in human iPSC-based models; neuron-glia interactions examines with in vitro co-culture; epigenetic changes in cultures neurons and astrocytes; rat brain slices; brain punching technique; and using microRNA for in vitro neurotoxicity testing and related disorders. In .Neuromethods?.series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory..?.Authoritative and cutting-edge, .Cell Culture Techniques, Second Edition. is a valuable resource for students and experiences researchers who are interested in learning more and making risk decisions in this evolving field..
出版日期Book 2019Latest edition
關鍵詞Neurotoxicity; in vitro; iPSCs; GCCP; Neural Stem Cells; Cell culture
版次2
doihttps://doi.org/10.1007/978-1-4939-9228-7
isbn_softcover978-1-4939-9230-0
isbn_ebook978-1-4939-9228-7Series ISSN 0893-2336 Series E-ISSN 1940-6045
issn_series 0893-2336
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
The information of publication is updating

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B. L. Kasiske,W. London,M. Ellisontrophysiology techniques demonstrate that the neuronal networks develop spontaneous activity, including synchronized calcium oscillations that coincide with spontaneous changes in membrane potential as well as spontaneous electrical activity with defined (network) bursting. Importantly, as shown in
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Eric P. Chassignet,Jacques Verronical systems can be used to enhance rigor and may include the analysis of the level of reactive oxygen/nitrogen species (RONS) present, the evaluation of cellular redox systems, and the modification of biomolecules. We describe here three such methods applied to stem cell-derived neurons: (1) the ch
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Ocean Modeling and Parameterizationhods to assess cell type-specific gene expression, glial-specific pharmacological inhibition, and genetic manipulation that can be used to evaluate glial reactivity, with the overall goal of defining the microglial and astroglial activation phenotype that results from exposures to broad classes of n
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Alexei A. Pevtsov,Richard C. Canfield spine formation. To this end, this chapter describes the procedures to set up a sandwich co-culture system from primary rat glial cells and hippocampal neurons, and highlights advantages and disadvantages of this approach and its possible application in the investigation of individual glial factor
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A Mechanism Driving Solar Flaresf signaling cascades on the outer mitochondrial membrane (OMM) can greatly influence organelle form and function. Thus, interpreting OMM signaling events in the context of mitochondrial function is critical to understanding the role of stress-responsive protein kinases and phosphatases in health and
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https://doi.org/10.1007/978-94-011-5220-4 describe methods for performing primary neuron and primary astrocyte cultures from rodents. We also provide methods for measuring DNA methylation (methylated DNA immunoprecipitation) and histone modifications (chromatin immunoprecipitation). Studies utilizing these methods have resulted in importan
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NVSS Observations of UGC Galaxiesin living animals. Here we describe several advantages of this preparation for simple acute neurotoxicological studies and some of the experimental approaches that, in our experience, can reveal, in a rapid manner, neurotoxic and neuroprotective profiles of several molecules in different acute toxic
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