Titlebook: Cell Culture Engineering IV; Improvements of Huma Barry C. Buckland,John G. Aunins,Weichang Zhou Book 1995 Springer Science+Business Media

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Applications of improved stoichiometric model in medium design and fed-batch cultivation of animal by 8-fold compared to fed-batch culture in T-flask using the previous stoichiometric model. Ammonia formation was also decreased compared with both the batch and fed-batch cultures. Most importantly, the monoclonal antibody concentration reached 900 mg l., an increase of 17- and 1.6-fold compared wi

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