| 書(shū)目名稱 | Celiac Disease | | 副標(biāo)題 | Methods and Protocol | | 編輯 | Anthony W. Ryan | | 視頻video | http://file.papertrans.cn/223/222723/222723.mp4 | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 叢書(shū)名稱 | Methods in Molecular Biology | | 圖書(shū)封面 |  | | 描述 | .This volume provides detailed molecular analysis of the associated pathways and cell types involved in Celiac Disease, and embraces new technologies such as next generation sequencing. The book is divided into three parts: Part I gives a background overview with a number of reviews to describe the history and nature of the disease, its diagnosis, the role of animal models, and study designs for investigating genetic susceptibility. Part II describes molecular techniques, including tissue culture, isolation and cloning of relevant cell types, high content analysis of biopsies, and HLA genotyping. The final part of the book begins with an outline of scripting for data management, focusing on tools that are freely available to researchers who wish to explore them. It also describes analysis pipelines for bioinformatics prediction of antigenicity, quality control and analysis of GWAS data, and transcriptome analysis by next generation sequencing. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, | | 出版日期 | Book 2015 | | 關(guān)鍵詞 | Coeliac Disease; HLA genotyping; auto-antigen; gene expression; generation sequencing; non-HLA loci; repor | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4939-2839-2 | | isbn_softcover | 978-1-4939-4775-1 | | isbn_ebook | 978-1-4939-2839-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media New York 2015 |
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